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human lung epithelial cell line beas2b  (ATCC)


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    ATCC human lung epithelial cell line beas2b
    TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in <t>BEAS2B,</t> HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.
    Human Lung Epithelial Cell Line Beas2b, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+epithelial+cell+line+beas2b/BEAS-2B/pmc12508908-53-1-20
    Average 99 stars, based on 4041 article reviews
    human lung epithelial cell line beas2b - by Bioz Stars, 2026-10
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    1) Product Images from "Integrated multi-omics analysis reveals TMEM147 as an immunosuppressive prognostic biomarker in LUAD"

    Article Title: Integrated multi-omics analysis reveals TMEM147 as an immunosuppressive prognostic biomarker in LUAD

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2025.102281

    TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in BEAS2B, HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.
    Figure Legend Snippet: TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in BEAS2B, HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.

    Techniques Used: Migration, In Vitro, Expressing, Western Blot, Control

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    Figure 4. ADAMTS1 promotes migration, invasion, and EMT of <t>BEAS2B</t> cells. ADAMTS1 overexpression plasmids or si-ADAMTS1 was transfected into BEAS2B cells. (A) Wound-healing assay was used for detecting the migration of BEAS2B cells transfected with ADAMTS1 overexpression plasmids. (B) Transwell examined the migration and invasion of BEAS2B cells (scale bar=50 μm). (C) Protein expressions of E- cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. NC=negative control. (D) The wound-healing assay showed the migration of BEAS2B cells after the knockdown of ADATMS1. (E) Transwell assays showed the migration and invasion of BEAS2B cells after the knockdown of ADATMS1 (scale bar=50 μm). (F) Protein expressions of E-cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. (G) EdU staining was used for detecting cell proliferation. Scale bar=100 μm. *P<0.05, **P<0.01, ***P<0.001 compared with NC or si-NC group. N=3.
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    TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in BEAS2B, HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Integrated multi-omics analysis reveals TMEM147 as an immunosuppressive prognostic biomarker in LUAD

    doi: 10.1016/j.bbrep.2025.102281

    Figure Lengend Snippet: TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in BEAS2B, HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.

    Article Snippet: The human lung epithelial cell line BEAS2B and four LUAD-derived cell lines (A549, NCI–H1299, HCC827 and NCI–H1975) were acquired from ATCC.

    Techniques: Migration, In Vitro, Expressing, Western Blot, Control

    Figure 4. ADAMTS1 promotes migration, invasion, and EMT of BEAS2B cells. ADAMTS1 overexpression plasmids or si-ADAMTS1 was transfected into BEAS2B cells. (A) Wound-healing assay was used for detecting the migration of BEAS2B cells transfected with ADAMTS1 overexpression plasmids. (B) Transwell examined the migration and invasion of BEAS2B cells (scale bar=50 μm). (C) Protein expressions of E- cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. NC=negative control. (D) The wound-healing assay showed the migration of BEAS2B cells after the knockdown of ADATMS1. (E) Transwell assays showed the migration and invasion of BEAS2B cells after the knockdown of ADATMS1 (scale bar=50 μm). (F) Protein expressions of E-cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. (G) EdU staining was used for detecting cell proliferation. Scale bar=100 μm. *P<0.05, **P<0.01, ***P<0.001 compared with NC or si-NC group. N=3.

    Journal: Aging

    Article Title: ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β.

    doi: 10.18632/aging.204594

    Figure Lengend Snippet: Figure 4. ADAMTS1 promotes migration, invasion, and EMT of BEAS2B cells. ADAMTS1 overexpression plasmids or si-ADAMTS1 was transfected into BEAS2B cells. (A) Wound-healing assay was used for detecting the migration of BEAS2B cells transfected with ADAMTS1 overexpression plasmids. (B) Transwell examined the migration and invasion of BEAS2B cells (scale bar=50 μm). (C) Protein expressions of E- cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. NC=negative control. (D) The wound-healing assay showed the migration of BEAS2B cells after the knockdown of ADATMS1. (E) Transwell assays showed the migration and invasion of BEAS2B cells after the knockdown of ADATMS1 (scale bar=50 μm). (F) Protein expressions of E-cadherin, N-cadherin, and Vimentin in BEAS2B cells were detected using Western blot. (G) EdU staining was used for detecting cell proliferation. Scale bar=100 μm. *P<0.05, **P<0.01, ***P<0.001 compared with NC or si-NC group. N=3.

    Article Snippet: NSCLC cell lines (A549 or H226) and human normal lung epithelial cell line BEAS2B cells were obtained from American Type Culture Collection (ATCC, USA).

    Techniques: Migration, Over Expression, Transfection, Wound Healing Assay, Western Blot, Negative Control, Knockdown, Staining